Knowledge mediation in software quality engineering

The risk of failure of the software development process remains high despite many attempts to improve the quality of software engineering. Contemporary approaches to process assurance, such as the capability maturity model have not prevented systemic failures, nor have project management methodologies provided guarantees of software quality. The paper proposes an approach to software quality assurance based on a knowledge mediated concurrent audit, which incorporates essential feedback processes. Through a tightly integrated approach to quality audit, programmers would be empowered to use any chosen methodology to advantage, supported by intelligent monitoring of the essential interactions which occur in the development process. An experimental application implementing some aspects of the proposal is described <br /

The Grizzly, October 26, 1979

J-Board Issues Warning • Graduate Aids NASA In Antimatter Finding • Campus Planning Group Looks At Student Life • Alumnus Honored for Middle East Peace Role • Letters to the Editor • Fashion Show In Wismer • Pianist to Perform at Ursinus Wins International Competition • College Bowl Update • Sports Profile: John Onopchenko • Rooters Lose, Rebound for Win • Videon Top Scorer On Third Team • Bear Pack Win Aided By Frosh • Hockey Aims For Berth • V-Ball Edges Swarthmorehttps://digitalcommons.ursinus.edu/grizzlynews/1025/thumbnail.jp

Effects of increasing the affinity of CarD for RNA polymerase on Mycobacterium tuberculosis growth, rRNA transcription, and virulence

CarD is an essential RNA polymerase (RNAP) interacting protein in Mycobacterium tuberculosis that stimulates formation of RNAP-promoter open complexes. CarD plays a complex role in M. tuberculosis growth and virulence that is not fully understood. Therefore, to gain further insight into the role of CarD in M. tuberculosis growth and virulence, we determined the effect of increasing the affinity of CarD for RNAP. Using site-directed mutagenesis guided by crystal structures of CarD bound to RNAP, we identified amino acid substitutions that increase the affinity of CarD for RNAP. Using these substitutions, we show that increasing the affinity of CarD for RNAP increases the stability of the CarD protein in M. tuberculosis. In addition, we show that increasing the affinity of CarD for RNAP increases the growth rate in M. tuberculosis without affecting 16S rRNA levels. We further show that increasing the affinity of CarD for RNAP reduces M. tuberculosis virulence in a mouse model of infection despite the improved growth rate in vitro. Our findings suggest that the CarD-RNAP interaction protects CarD from proteolytic degradation in M. tuberculosis, establish that growth rate and rRNA levels can be uncoupled in M. tuberculosis and demonstrate that the strength of the CarD-RNAP interaction has been finely tuned to optimize virulence. IMPORTANCE Mycobacterium tuberculosis, the causative agent of tuberculosis, remains a major global health problem. In order to develop new strategies to battle this pathogen, we must gain a better understanding of the molecular processes involved in its survival and pathogenesis. We have previously identified CarD as an essential transcriptional regulator in mycobacteria. In this study, we detail the effects of increasing the affinity of CarD for RNAP on transcriptional regulation, CarD protein stability, and virulence. These studies expand our understanding of the global transcription regulator CarD, provide insight into how CarD activity is regulated, and broaden our understanding of prokaryotic transcription

The Grizzly, April 25, 1980

Campus Intruder Apprehended • Spring Fling Slated Tomorrow • Art Piece Missing • Campus Survey Shows Strong Contrast With Pennsylvania Primary Results • UC in Judo Tournament • Fun Run for Charity • Appeal for Jail Visitors • Sun Shines on Parents\u27 Day • Art in the Afternoon • Ursinus Springs Into Springtime • Summer is not Far Behind • Student Art Exhibit Opens • Progress at Ritter Center • Dr. Parsons Speaks at UCC College Heritage Day • Superstars 1980 • Men\u27s Lacrosse Shoots for a Winning Season • Sports Profile: Ken West • Thinclads Up Slate to 6-2 • Softball Shows Great Improvement • Baseball Playoff Picture Looks Dim • Women\u27s Lacrosse Maintains Traditionhttps://digitalcommons.ursinus.edu/grizzlynews/1039/thumbnail.jp

The Grizzly, October 31, 1980

Campus Poll Favors Reagan, Anderson • Sorority Pleads Guilty To Alcohol Violations • Board Approves Cost Increases For Next Year • Carter & Reagan Make Final Philly Appearances • Athletic Hall of Fame Slated For Founder\u27s Day • It\u27s Laurie for Holmecoming Queen 1980 • Utility Gym Opens With Exciting Concert • Richter Hosts Town Meeting • APO Charter Reviewed • Halloween Dates Back 2000 Years • Booters Drop Three Straight • Gridders Drowned By Swarthmore • Hockey Suffers Season\u27s Second Defeat • Homecoming Sweet For Cross Countryhttps://digitalcommons.ursinus.edu/grizzlynews/1045/thumbnail.jp

The Grizzly, November 2, 1979

TCE Level Too High? Collegeville Water Poisoning Level Questioned • Greaseband to Present Return Performance In Helfferich • Senior Plays Role As Big Brother • Letters to the Editor • Roving Reporter: Infirmary services • Tull: Another Visit From The Past • Music News: Foreigner, Tom Petty • The Best Of The Seventies - Part 2 • Homecoming 1979 • Volleyball Team Continues Up And Down • Hockey\u27s 3 & 4 Finish Season • JV Hockey Ties Rams, 1-1 • Gridders Upset Dutchmen • Five Honored • Cross Country Wins Two More • Espo Paces Booter Win • Hockey Edges Rivalshttps://digitalcommons.ursinus.edu/grizzlynews/1026/thumbnail.jp

The Grizzly, March 28, 1980

Campus Visitor Assaulted • Two Students Found Guilty Of Violations • Committee Studies Frats\u27 Academics • Lantern Seeks Editor • Administration Apathy or Illiteracy? • TKE Sponsors X-Country Keg Roll • USGA Notes • President Urges Sidewalk Use • USGA Candidates\u27 Platforms • Repko\u27s Review • Interest inventory to be given again • Checking By Mail • Jazz Coffeehouse At The Union • Portrait Of A Professor: Dr. S. Ross Doughty • Survey Taken Of Class Of \u2779 • Earl Awarded at Evening School • Whitians Chosen • Bears Win Home Opener • Spring Track Starts Tomorrow • Slow Start For Men\u27s Lacrossehttps://digitalcommons.ursinus.edu/grizzlynews/1037/thumbnail.jp

The Grizzly, December 7, 1979

Faculty Approves Combined Majors • C&C Offered Next Semester • College Applies To Phi Beta Kappa • Vandalism Strikes Beardwood • Letters to the Editor • Roving Reporter: Final exams • Timely Displays Of Popular American Feeling • Music News: The Year In Music • Ursinus To Present Messiah • Alumnus Donates Sculpture • Friends Of The Library Hold Fall Meeting • College Institutes Math/Econ Program • Swimmers Rebound To Notch Initial Win • Penalty Flicks Hurt Hockey • Volleyball Club Shows Progresshttps://digitalcommons.ursinus.edu/grizzlynews/1030/thumbnail.jp

Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification

Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification

The Grizzly, February 13, 1981

Water Crisis Worsens: TCE Also a Problem • Pett Leaving Economics Department • Mobley Breaks Basketball Record • News Briefs: Meistersingers concert in Oreland; Ursinus professor published • Departmental Focus: English Department; History Department • Orientation Committee Applications Being Taken • 1981 Fraternity Pledge Classes • Music News • Female Artists Topic of Forum • Coffeehouse: A Folk Touch • Candidates for USGA Offices • Women\u27s B-Ball Undefeated 9 Straight • Tight Season Ahead for Intramural B-Ball • Badminton Team Playin\u27 Tough • Hoopsters Claim Two More Victims • Grizzly Grapplers Mark at 7-4-1 • Aquabears Dunk Foes: Swimmers Living Up to .500 Goalhttps://digitalcommons.ursinus.edu/grizzlynews/1052/thumbnail.jp